Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor.

نویسندگان

  • M A Cantrell
  • D Anderson
  • D P Cerretti
  • V Price
  • K McKereghan
  • R J Tushinski
  • D Y Mochizuki
  • A Larsen
  • K Grabstein
  • S Gillis
چکیده

Human granulocyte/macrophage colony-stimulating factor (GM-CSF) is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. In this report we have used a mouse GM-CSF cDNA clone to isolate human GM-CSF clones from libraries made from HUT-102 messenger RNA and mitogen-stimulated T-lymphocyte messenger RNA. The human cDNA clones contained a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and showed 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. One of these cDNA clones was shown to direct the synthesis of biologically active GM-CSF using a yeast expression system. The gene for human GM-CSF appears to exist as a single-copy gene.

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عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 82 18  شماره 

صفحات  -

تاریخ انتشار 1985